Secondary metabolite biosynthetic diversity in the fungal family Hypoxylaceae and Xylaria hypoxylon.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractTo date little is known about the genetic background that drives the production and diversification of secondary metabolites in the Hypoxylaceae. With the recent availability of high-quality genome sequences for 13 representative species and one relative (Xylaria hypoxylon) we attempted to survey the diversity of biosynthetic pathways in these organisms to investigate their true potential as secondary metabolite producers. Manual search strategies based on the accumulated knowledge on biosynthesis in fungi enabled us to identify 783 biosynthetic pathways across 14 studied species, the majority of which were arranged in biosynthetic gene clusters (BGC). The similarity of BGCs was analysed with the BiG-SCAPE engine which organised the BGCs into 375 gene cluster families (GCF). Only ten GCFs were conserved across all of these fungi indicating that speciation is accompanied by changes in secondary metabolism. From the known compounds produced by the family members some can be directly correlated with identified BGCs which is highlighted herein by the azaphilone, dihydroxynaphthalene, tropolone, cytochalasan, terrequinone, terphenyl and brasilane pathways giving insights into the evolution and diversification of those compound classes. Vice versa, products of various BGCs can be predicted through homology analysis with known pathways from other fungi as shown for the identified ergot alkaloid, trigazaphilone, curvupallide, viridicatumtoxin and swainsonine BGCs. However, the majority of BGCs had no obvious links to known products from the Hypoxylaceae or other well-studied biosynthetic pathways from fungi. These findings highlight that the number of known compounds strongly underrepresents the biosynthetic potential in these fungi and that a tremendous number of unidentified secondary metabolites is still hidden. Moreover, with increasing numbers of genomes for further Hypoxylaceae species becoming available, the likelihood of revealing new biosynthetic pathways that encode new, potentially useful compounds will significantly improve. Reaching a better understanding of the biology of these producers, and further development of genetic methods for their manipulation, will be crucial to access their treasures.
CitationStud Mycol. 2021 Aug 26;99:100118. doi: 10.1016/j.simyco.2021.100118.
AffiliationHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.
JournalStudies in mycology
The following license files are associated with this item:
- Creative Commons
- An interpreted atlas of biosynthetic gene clusters from 1,000 fungal genomes.
- Authors: Robey MT, Caesar LK, Drott MT, Keller NP, Kelleher NL
- Issue date: 2021 May 11
- Comparative Genomics Reveals a Remarkable Biosynthetic Potential of the <i>Streptomyces</i> Phylogenetic Lineage Associated with Rugose-Ornamented Spores.
- Authors: Chung YH, Kim H, Ji CH, Je HW, Lee D, Shim SH, Joo HS, Kang HS
- Issue date: 2021 Aug 31
- BiG-SLiCE: A highly scalable tool maps the diversity of 1.2 million biosynthetic gene clusters.
- Authors: Kautsar SA, van der Hooft JJJ, de Ridder D, Medema MH
- Issue date: 2021 Jan 13
- BiG-FAM: the biosynthetic gene cluster families database.
- Authors: Kautsar SA, Blin K, Shaw S, Weber T, Medema MH
- Issue date: 2021 Jan 8
- Phylogenetic Distribution of Secondary Metabolites in the Bacillus subtilis Species Complex.
- Authors: Steinke K, Mohite OS, Weber T, Kovács ÁT
- Issue date: 2021 Mar 9