Show simple item record

dc.contributor.authorBünsow, Dorothea
dc.contributor.authorTantawy, Eshraq
dc.contributor.authorOstermeier, Tjorven
dc.contributor.authorBähre, Heike
dc.contributor.authorGarbe, Annette
dc.contributor.authorLarsen, Jesper
dc.contributor.authorWinstel, Volker
dc.date.accessioned2021-10-01T14:11:59Z
dc.date.available2021-10-01T14:11:59Z
dc.date.issued2021-03-29
dc.identifier.citationVirulence. 2021 Dec;12(1):989-1002. doi: 10.1080/21505594.2021.1903691.en_US
dc.identifier.pmid33779509
dc.identifier.doi10.1080/21505594.2021.1903691
dc.identifier.urihttp://hdl.handle.net/10033/623057
dc.description.abstractMethicillin-resistant Staphylococcus pseudintermedius (MRSP) is an emerging zoonotic pathogen of canine origin that causes an array of fatal diseases, including bacteremia and endocarditis. Despite large-scale genome sequencing projects have gained substantial insights into the genomic landscape of MRSP, current knowledge on virulence determinants that contribute to S. pseudintermedius pathogenesis during human or canine infection is very limited. Using a panel of genetically engineered MRSP variants and a mouse abscess model, we here identified the major secreted nuclease of S. pseudintermedius designated NucB and adenosine synthase A (AdsA) as two synergistically acting enzymes required for MRSP pathogenesis. Similar to Staphylococcus aureus, S. pseudintermedius requires nuclease secretion along with the activity of AdsA to degrade mammalian DNA for subsequent biosynthesis of cytotoxic deoxyadenosine. In this manner, S. pseudintermedius selectively kills macrophages during abscess formation thereby antagonizing crucial host immune cell responses. Ultimately, bioinformatics analyses revealed that NucB and AdsA are widespread in the global S. pseudintermedius population. Together, these data suggest that S. pseudintermedius deploys the canonical Nuc/AdsA pathway to persist during invasive disease and may aid in the development of new therapeutic strategies to combat infections caused by MRSP.en_US
dc.language.isoenen_US
dc.publisherTaylor & Francisen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectStaphylococcus pseudintermediusen_US
dc.subjectabscessen_US
dc.subjectadenosine synthase Aen_US
dc.subjectdeoxyadenosineen_US
dc.subjectnucleaseen_US
dc.titleMethicillin-resistant Staphylococcus pseudintermedius synthesizes deoxyadenosine to cause persistent infection.en_US
dc.typeArticleen_US
dc.typeen_US
dc.identifier.eissn2150-5608
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalVirulenceen_US
dc.source.volume12
dc.source.issue1
dc.source.beginpage989
dc.source.endpage1002
refterms.dateFOA2021-10-01T14:12:00Z
dc.source.journaltitleVirulence
dc.source.countryUnited States


Files in this item

Thumbnail
Name:
Bünsow et al.pdf
Size:
2.211Mb
Format:
PDF
Description:
Open Access publication
Thumbnail
Name:
Büsow_suppl.pdf
Size:
367.8Kb
Format:
PDF
Description:
supplemental material

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International