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dc.contributor.authorKotov, Vadim
dc.contributor.authorLunelli, Michele
dc.contributor.authorWald, Jiri
dc.contributor.authorKolbe, Michael
dc.contributor.authorMarlovits, Thomas C
dc.date.accessioned2021-10-04T13:09:39Z
dc.date.available2021-10-04T13:09:39Z
dc.date.issued2021-06-27
dc.identifier.citationBiochem Biophys Rep. 2021 Jun 27;27:101039. doi: 10.1016/j.bbrep.2021.101039.en_US
dc.identifier.pmid34258394
dc.identifier.doi10.1016/j.bbrep.2021.101039
dc.identifier.urihttp://hdl.handle.net/10033/623059
dc.description.abstractGram-negative pathogens evolved a syringe-like nanomachine, termed type 3 secretion system, to deliver protein effectors into the cytoplasm of host cells. An essential component of this system is a long helical needle filament that protrudes from the bacterial surface and connects the cytoplasms of the bacterium and the eukaryotic cell. Previous structural research was predominantly focused on reconstituted type 3 needle filaments, which lacked the biological context. In this work we introduce a facile procedure to obtain high-resolution cryo-EM structure of needle filaments attached to the basal body of type 3 secretion systems. We validate our approach by solving the structure of Salmonella PrgI filament and demonstrate its utility by obtaining the first high-resolution cryo-EM reconstruction of Shigella MxiH filament. Our work paves the way to systematic structural characterization of attached type 3 needle filaments in the context of mutagenesis studies, protein structural evolution and drug development.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCryo electron microscopyen_US
dc.subjectHelical reconstructionen_US
dc.subjectNeedle filamenten_US
dc.subjectSalmonella, host-pathogen interactionen_US
dc.subjectShigellaen_US
dc.subjectType 3 secretion systemen_US
dc.titleHelical reconstruction of and needle filaments attached to type 3 basal bodies.en_US
dc.typeArticleen_US
dc.identifier.eissn2405-5808
dc.contributor.departmentCSSB, Centre for Structural Systembiologie, Notkestr.85, 22607 Hamburg. Germany.en_US
dc.identifier.journalBiochemistry and biophysics reportsen_US
dc.source.volume27
dc.source.beginpage101039
dc.source.endpage
refterms.dateFOA2021-10-04T13:09:40Z
dc.source.journaltitleBiochemistry and biophysics reports
dc.source.countryNetherlands


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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International