Helical reconstruction of and needle filaments attached to type 3 basal bodies.
dc.contributor.author | Kotov, Vadim | |
dc.contributor.author | Lunelli, Michele | |
dc.contributor.author | Wald, Jiri | |
dc.contributor.author | Kolbe, Michael | |
dc.contributor.author | Marlovits, Thomas C | |
dc.date.accessioned | 2021-10-04T13:09:39Z | |
dc.date.available | 2021-10-04T13:09:39Z | |
dc.date.issued | 2021-06-27 | |
dc.identifier.citation | Biochem Biophys Rep. 2021 Jun 27;27:101039. doi: 10.1016/j.bbrep.2021.101039. | en_US |
dc.identifier.pmid | 34258394 | |
dc.identifier.doi | 10.1016/j.bbrep.2021.101039 | |
dc.identifier.uri | http://hdl.handle.net/10033/623059 | |
dc.description.abstract | Gram-negative pathogens evolved a syringe-like nanomachine, termed type 3 secretion system, to deliver protein effectors into the cytoplasm of host cells. An essential component of this system is a long helical needle filament that protrudes from the bacterial surface and connects the cytoplasms of the bacterium and the eukaryotic cell. Previous structural research was predominantly focused on reconstituted type 3 needle filaments, which lacked the biological context. In this work we introduce a facile procedure to obtain high-resolution cryo-EM structure of needle filaments attached to the basal body of type 3 secretion systems. We validate our approach by solving the structure of Salmonella PrgI filament and demonstrate its utility by obtaining the first high-resolution cryo-EM reconstruction of Shigella MxiH filament. Our work paves the way to systematic structural characterization of attached type 3 needle filaments in the context of mutagenesis studies, protein structural evolution and drug development. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Cryo electron microscopy | en_US |
dc.subject | Helical reconstruction | en_US |
dc.subject | Needle filament | en_US |
dc.subject | Salmonella, host-pathogen interaction | en_US |
dc.subject | Shigella | en_US |
dc.subject | Type 3 secretion system | en_US |
dc.title | Helical reconstruction of and needle filaments attached to type 3 basal bodies. | en_US |
dc.type | Article | en_US |
dc.identifier.eissn | 2405-5808 | |
dc.contributor.department | CSSB, Centre for Structural Systembiologie, Notkestr.85, 22607 Hamburg. Germany. | en_US |
dc.identifier.journal | Biochemistry and biophysics reports | en_US |
dc.source.volume | 27 | |
dc.source.beginpage | 101039 | |
dc.source.endpage | ||
refterms.dateFOA | 2021-10-04T13:09:40Z | |
dc.source.journaltitle | Biochemistry and biophysics reports | |
dc.source.country | Netherlands |