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dc.contributor.authorPommerenke, Claudia
dc.contributor.authorRand, Ulfert
dc.contributor.authorUphoff, Cord C
dc.contributor.authorNagel, Stefan
dc.contributor.authorZaborski, Margarete
dc.contributor.authorHauer, Vivien
dc.contributor.authorKaufmann, Maren
dc.contributor.authorMeyer, Corinna
dc.contributor.authorDenkmann, Sabine A
dc.contributor.authorRiese, Peggy
dc.contributor.authorEschke, Kathrin
dc.contributor.authorKim, Yeonsu
dc.contributor.authorSafranko, Zeljka Macak
dc.contributor.authorKurolt, Ivan-Christian
dc.contributor.authorMarkotic, Alemka
dc.contributor.authorCicin-Sain, Luka
dc.contributor.authorSteenpass, Laura
dc.date.accessioned2021-10-08T08:40:48Z
dc.date.available2021-10-08T08:40:48Z
dc.date.issued2021-08-02
dc.identifier.citationPLoS One. 2021 Aug 2;16(8):e0255622. doi: 10.1371/journal.pone.0255622.en_US
dc.identifier.pmid34339474
dc.identifier.doi10.1371/journal.pone.0255622
dc.identifier.urihttp://hdl.handle.net/10033/623066
dc.description.abstractThe SARS-CoV-2 pandemic is a major global threat that sparked global research efforts. Pre-clinical and biochemical SARS-CoV-2 studies firstly rely on cell culture experiments where the importance of choosing an appropriate cell culture model is often underestimated. We here present a bottom-up approach to identify suitable permissive cancer cell lines for drug screening and virus research. Human cancer cell lines were screened for the SARS-CoV-2 cellular entry factors ACE2 and TMPRSS2 based on RNA-seq data of the Cancer Cell Line Encyclopedia (CCLE). However, experimentally testing permissiveness towards SARS-CoV-2 infection, we found limited correlation between receptor expression and permissiveness. This underlines that permissiveness of cells towards viral infection is determined not only by the presence of entry receptors but is defined by the availability of cellular resources, intrinsic immunity, and apoptosis. Aside from established cell culture infection models CACO-2 and CALU-3, three highly permissive human cell lines, colon cancer cell lines CL-14 and CL-40 and the breast cancer cell line CAL-51 and several low permissive cell lines were identified. Cell lines were characterised in more detail offering a broader choice of non-overexpression in vitro infection models to the scientific community. For some cell lines a truncated ACE2 mRNA and missense variants in TMPRSS2 might hint at disturbed host susceptibility towards viral entry.en_US
dc.language.isoenen_US
dc.publisherPLOSen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshAngiotensin-Converting Enzyme 2 / geneticsen_US
dc.subject.meshAngiotensin-Converting Enzyme 2 / metabolismen_US
dc.subject.meshCOVID-19 / virology*en_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshHumansen_US
dc.subject.meshReceptors, Virus* / geneticsen_US
dc.subject.meshReceptors, Virus* / metabolismen_US
dc.subject.meshSARS-CoV-2 / physiology*en_US
dc.subject.meshSerine Endopeptidases / geneticsen_US
dc.subject.meshSerine Endopeptidases / metabolismen_US
dc.subject.meshVirus Internalization*en_US
dc.titleIdentification of cell lines CL-14, CL-40 and CAL-51 as suitable models for SARS-CoV-2 infection studies.en_US
dc.typeArticleen_US
dc.identifier.eissn1932-6203
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalPloS oneen_US
dc.source.volume16
dc.source.issue8
dc.source.beginpagee0255622
dc.source.endpage
refterms.dateFOA2021-10-08T08:40:49Z
dc.source.journaltitlePloS one
dc.source.countryUnited States


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International