Show simple item record

dc.contributor.authorLettieri, Giulia Melo
dc.contributor.authorSantiago, Luander Medrado
dc.contributor.authorLettieri, Giancarlo Crosara
dc.contributor.authorBorges, Luiz Gustavo Dos Anjos
dc.contributor.authorMarconatto, Letícia
dc.contributor.authorde Oliveira, Laudimar Alves
dc.contributor.authorDamé-Teixeira, Nailê
dc.contributor.authorSalles, Loise Pedrosa
dc.date.accessioned2021-10-28T12:46:07Z
dc.date.available2021-10-28T12:46:07Z
dc.date.issued2021-08-26
dc.identifier.citationFront Cell Infect Microbiol. 2021 Aug 26;11:720790. doi: 10.3389/fcimb.2021.720790.en_US
dc.identifier.pmid34513733
dc.identifier.doi10.3389/fcimb.2021.720790
dc.identifier.urihttp://hdl.handle.net/10033/623085
dc.description.abstractPapillon-Lefèvre syndrome (PLS) is an autosomal recessive rare disease, main characteristics of which include palmoplantar hyperkeratosis and premature edentulism due to advanced periodontitis (formerly aggressive periodontitis). This study aimed to characterize the oral phenotype, including salivary parameters, and the salivary microbiome of three PLS sisters, comparatively. Two sisters were toothless (PLSTL1 and PLSTL2), and one sister had most of the teeth in the oral cavity (PLST). Total DNA was extracted from the unstimulated saliva, and the amplicon sequencing of the 16S rRNA gene fragment was performed in an Ion PGM platform. The amplicon sequence variants (ASVs) were obtained using the DADA2 pipeline, and the taxonomy was assigned using the SILVA v.138. The main phenotypic characteristics of PLS were bone loss and premature loss of primary and permanent dentition. The PLST sister presented advanced periodontitis with gingival bleeding and suppuration, corresponding to the advanced periodontitis as a manifestation of systemic disease, stage IV, grade C. All three PLS sisters presented hyposalivation as a possible secondary outcome of the syndrome. Interestingly, PLST salivary microbiota was dominated by the uncultured bacteria Bacterioidales (F0058), Fusobacterium, Treponema, and Sulfophobococcus (Archaea domain). Streptococcus, Haemophilus, and Caldivirga (Archaea) dominated the microbiome of the PLSTL1 sister, while the PLSTL2 had higher abundances of Lactobacillus and Porphyromonas. This study was the first to show a high abundance of organisms belonging to the Archaea domain comprising a core microbiome in human saliva. In conclusion, a PLST individual does have a microbiota different from that of the periodontitis' aggressiveness previously recognized. Due to an ineffective cathepsin C, the impairment of neutrophils probably provided a favorable environment for the PLS microbiome. The interactions of Bacteroidales F0058, Caldivirga, and Sulfophobococcus with the microbial consortium of PLS deserves future investigation. Traditional periodontal therapy is not efficient in PLS patients. Unraveling the PLS microbiome is essential in searching for appropriate treatment and avoiding early tooth loss.en_US
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectPapillon–Lefèvre diseaseen_US
dc.subjectcathepsin Cen_US
dc.subjectmicrobiologyen_US
dc.subjectperiodontal infectionen_US
dc.subjectperiodontitisen_US
dc.subjectsalivaen_US
dc.titleOral Phenotype and Salivary Microbiome of Individuals With Papillon-Lefèvre Syndrome.en_US
dc.typeArticleen_US
dc.identifier.eissn2235-2988
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalFrontiers in cellular and infection microbiologyen_US
dc.source.volume11
dc.source.beginpage720790
dc.source.endpage
refterms.dateFOA2021-10-28T12:46:07Z
dc.source.journaltitleFrontiers in cellular and infection microbiology
dc.source.countrySwitzerland


Files in this item

Thumbnail
Name:
Letteri et al.pdf
Size:
1.679Mb
Format:
PDF
Description:
Open Access publication

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International