A role for PchHI as the ABC transporter in iron acquisition by the siderophore pyochelin in Pseudomonas aeruginosa.
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Authors
Roche, BéatriceGarcia-Rivera, Mariel A
Normant, Vincent
Kuhn, Lauriane
Hammann, Philippe
Brönstrup, Mark
Mislin, Gaëtan L A
Schalk, Isabelle J
Issue Date
2021-10-18
Metadata
Show full item recordAbstract
Iron is an essential nutrient for bacterial growth but poorly bioavailable. Bacteria scavenge ferric iron by synthesizing and secreting siderophores, small compounds with a high affinity for iron. Pyochelin (PCH) is one of the two siderophores produced by the opportunistic pathogen Pseudomonas aeruginosa. After capturing a ferric iron molecule, PCH-Fe is imported back into bacteria first by the outer membrane transporter FptA and then by the inner membrane permease FptX. Here, using molecular biology, 55 Fe uptake assays, and LC-MS/MS quantification, we first find a role for PchHI as the heterodimeric ABC transporter involved in the siderophore-free iron uptake into the bacterial cytoplasm. We also provide the first evidence that PCH is able to reach the bacterial periplasm and cytoplasm when both FptA and FptX are expressed. Finally, we detected an interaction between PchH and FptX, linking the ABC transporter PchHI with the inner permease FptX in the PCH-Fe uptake pathway. These results pave the way for a better understanding of the PCH siderophore pathway, giving future directions to tackle P. aeruginosa infections.Citation
Environ Microbiol. 2021 Oct 18. doi: 10.1111/1462-2920.15811. Epub ahead of print.Affiliation
HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.Publisher
John Wiley & Sons LTDJournal
Environmental microbiologyPubMed ID
34664350Type
ArticleLanguage
enEISSN
1462-2920ae974a485f413a2113503eed53cd6c53
10.1111/1462-2920.15811
Scopus Count
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- Creative Commons