Show simple item record

dc.contributor.authorRoche, Béatrice
dc.contributor.authorGarcia-Rivera, Mariel A
dc.contributor.authorNormant, Vincent
dc.contributor.authorKuhn, Lauriane
dc.contributor.authorHammann, Philippe
dc.contributor.authorBrönstrup, Mark
dc.contributor.authorMislin, Gaëtan L A
dc.contributor.authorSchalk, Isabelle J
dc.date.accessioned2021-12-07T14:20:21Z
dc.date.available2021-12-07T14:20:21Z
dc.date.issued2021-10-18
dc.identifier.citationEnviron Microbiol. 2021 Oct 18. doi: 10.1111/1462-2920.15811. Epub ahead of print.en_US
dc.identifier.pmid34664350
dc.identifier.doi10.1111/1462-2920.15811
dc.identifier.urihttp://hdl.handle.net/10033/623115
dc.description.abstractIron is an essential nutrient for bacterial growth but poorly bioavailable. Bacteria scavenge ferric iron by synthesizing and secreting siderophores, small compounds with a high affinity for iron. Pyochelin (PCH) is one of the two siderophores produced by the opportunistic pathogen Pseudomonas aeruginosa. After capturing a ferric iron molecule, PCH-Fe is imported back into bacteria first by the outer membrane transporter FptA and then by the inner membrane permease FptX. Here, using molecular biology, 55 Fe uptake assays, and LC-MS/MS quantification, we first find a role for PchHI as the heterodimeric ABC transporter involved in the siderophore-free iron uptake into the bacterial cytoplasm. We also provide the first evidence that PCH is able to reach the bacterial periplasm and cytoplasm when both FptA and FptX are expressed. Finally, we detected an interaction between PchH and FptX, linking the ABC transporter PchHI with the inner permease FptX in the PCH-Fe uptake pathway. These results pave the way for a better understanding of the PCH siderophore pathway, giving future directions to tackle P. aeruginosa infections.en_US
dc.language.isoenen_US
dc.publisherJohn Wiley & Sons LTDen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleA role for PchHI as the ABC transporter in iron acquisition by the siderophore pyochelin in Pseudomonas aeruginosa.en_US
dc.typeArticleen_US
dc.identifier.eissn1462-2920
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalEnvironmental microbiologyen_US
refterms.dateFOA2021-12-07T14:20:21Z
dc.source.journaltitleEnvironmental microbiology
dc.source.countryEngland


Files in this item

Thumbnail
Name:
Roche et al.pdf
Size:
835.8Kb
Format:
PDF
Description:
Open Access article

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International