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dc.contributor.authorCarius, Patrick
dc.contributor.authorDubois, Aurélie
dc.contributor.authorAjdarirad, Morvarid
dc.contributor.authorArtzy-Schnirman, Arbel
dc.contributor.authorSznitman, Josué
dc.contributor.authorSchneider-Daum, Nicole
dc.contributor.authorLehr, Claus-Michael
dc.date.accessioned2021-12-13T14:22:30Z
dc.date.available2021-12-13T14:22:30Z
dc.date.issued2021-10-06
dc.identifier.citationFront Bioeng Biotechnol. 2021 Oct 6;9:743236. doi: 10.3389/fbioe.2021.743236.en_US
dc.identifier.issn2296-4185
dc.identifier.pmid34692661
dc.identifier.doi10.3389/fbioe.2021.743236
dc.identifier.urihttp://hdl.handle.net/10033/623119
dc.description.abstractComplex in vitro models, especially those based on human cells and tissues, may successfully reduce or even replace animal models within pre-clinical development of orally inhaled drug products. Microfluidic lung-on-chips are regarded as especially promising models since they allow the culture of lung specific cell types under physiological stimuli including perfusion and air-liquid interface (ALI) conditions within a precisely controlled in vitro environment. Currently, though, such models are not available to a broad user community given their need for sophisticated microfabrication techniques. They further require systematic comparison to well-based filter supports, in analogy to traditional Transwells®. We here present a versatile perfusable platform that combines the advantages of well-based filter supports with the benefits of perfusion, to assess barrier permeability of and aerosol deposition on ALI cultured pulmonary epithelial cells. The platform as well as the required technical accessories can be reproduced via a detailed step-by-step protocol and implemented in typical bio-/pharmaceutical laboratories without specific expertise in microfabrication methods nor the need to buy costly specialized equipment. Calu-3 cells cultured under liquid covered conditions (LCC) inside the platform showed similar development of transepithelial electrical resistance (TEER) over a period of 14 days as cells cultured on a traditional Transwell®. By using a customized deposition chamber, fluorescein sodium was nebulized via a clinically relevant Aerogen® Solo nebulizer onto Calu-3 cells cultured under ALI conditions within the platform. This not only allowed to analyze the transport of fluorescein sodium after ALI deposition under perfusion, but also to compare it to transport under traditional static conditions.en_US
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectaerosol depositionen_US
dc.subjectair-liquid interface (ALI)en_US
dc.subjectdrug testingen_US
dc.subjectperfusionen_US
dc.subjectpermeabilityen_US
dc.subjectpulmonary epitheliaen_US
dc.subjecttransepithelial electrical resistance (TEER)en_US
dc.titlePerfuPul-A Versatile Perfusable Platform to Assess Permeability and Barrier Function of Air Exposed Pulmonary Epithelia.en_US
dc.typeArticleen_US
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1, 66123 Saarbrücken, Germany.en_US
dc.identifier.journalFrontiers in bioengineering and biotechnologyen_US
dc.source.volume9
dc.source.beginpage743236
dc.source.endpage
refterms.dateFOA2021-12-13T14:22:31Z
dc.source.journaltitleFrontiers in bioengineering and biotechnology
dc.source.countrySwitzerland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International