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dc.contributor.authorHill, Chris H
dc.contributor.authorPekarek, Lukas
dc.contributor.authorNapthine, Sawsan
dc.contributor.authorKibe, Anuja
dc.contributor.authorFirth, Andrew E
dc.contributor.authorGraham, Stephen C
dc.contributor.authorCaliskan, Neva
dc.contributor.authorBrierley, Ian
dc.date.accessioned2022-01-07T15:28:44Z
dc.date.available2022-01-07T15:28:44Z
dc.date.issued2021-12-09
dc.identifier.citationNat Commun. 2021 Dec 9;12(1):7166. doi: 10.1038/s41467-021-27400-7.en_US
dc.identifier.pmid34887415
dc.identifier.doi10.1038/s41467-021-27400-7
dc.identifier.urihttp://hdl.handle.net/10033/623133
dc.description.abstractrogrammed -1 ribosomal frameshifting (PRF) in cardioviruses is activated by the 2A protein, a multi-functional virulence factor that also inhibits cap-dependent translational initiation. Here we present the X-ray crystal structure of 2A and show that it selectively binds to a pseudoknot-like conformation of the PRF stimulatory RNA element in the viral genome. Using optical tweezers, we demonstrate that 2A stabilises this RNA element, likely explaining the increase in PRF efficiency in the presence of 2A. Next, we demonstrate a strong interaction between 2A and the small ribosomal subunit and present a cryo-EM structure of 2A bound to initiated 70S ribosomes. Multiple copies of 2A bind to the 16S rRNA where they may compete for binding with initiation and elongation factors. Together, these results define the structural basis for RNA recognition by 2A, show how 2A-mediated stabilisation of an RNA pseudoknot promotes PRF, and reveal how 2A accumulation may shut down translation during virus infection. © 2021. The Auen_US
dc.language.isoenen_US
dc.publisherNature Publishing Group (NPG)en_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleStructural and molecular basis for Cardiovirus 2A protein as a viral gene expression switch.en_US
dc.typeArticleen_US
dc.identifier.eissn2041-1723
dc.contributor.departmentHIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Shneider Strasse 2, 97080 Würzburg, Germany.en_US
dc.identifier.journalNature communicationsen_US
dc.source.volume12
dc.source.issue1
dc.source.beginpage7166
dc.source.endpage
refterms.dateFOA2022-01-07T15:28:44Z
dc.source.journaltitleNature communications
dc.source.countryUnited Kingdom
dc.source.countryUnited Kingdom
dc.source.countryUnited Kingdom
dc.source.countryEngland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International