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dc.contributor.authorLi, Jing
dc.contributor.authorGálvez, Eric J C
dc.contributor.authorAmend, Lena
dc.contributor.authorAlmási, Éva
dc.contributor.authorIljazovic, Aida
dc.contributor.authorLesker, Till R
dc.contributor.authorBielecka, Agata A
dc.contributor.authorSchorr, Eva-Magdalena
dc.contributor.authorStrowig, Till
dc.date.accessioned2022-01-11T14:39:34Z
dc.date.available2022-01-11T14:39:34Z
dc.date.issued2021-10-21
dc.identifier.citationEMBO J. 2021 Dec 1;40(23):e108287. doi: 10.15252/embj.2021108287. Epub 2021 Oct 21.en_US
dc.identifier.pmid34676563
dc.identifier.doi10.15252/embj.2021108287
dc.identifier.urihttp://hdl.handle.net/10033/623137
dc.description.abstractPrevotella copri is a prevalent inhabitant of the human gut and has been associated with plant-rich diet consumption and diverse health states. The underlying genetic basis of these associations remains enigmatic due to the lack of genetic tools. Here, we developed a novel versatile genetic toolbox for rapid and efficient genetic insertion and allelic exchange applicable to P. copri strains from multiple clades. Enabled by the genetic platform, we systematically investigated the specificity of polysaccharide utilization loci (PULs) and identified four highly conserved PULs for utilizing arabinan, pectic galactan, arabinoxylan, and inulin, respectively. Further genetic and functional analysis of arabinan utilization systems illustrate that P. copri has evolved two distinct types of arabinan-processing PULs (PULAra ) and that the type-II PULAra is significantly enriched in individuals consuming a vegan diet compared to other diets. In summary, this genetic toolbox will enable functional genetic studies for P. copri in future.en_US
dc.language.isoenen_US
dc.publisherEMBO Pressen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectPrevotella coprien_US
dc.subjectgenetic manipulationen_US
dc.subjecthuman dieten_US
dc.subjecthybrid two-component systemen_US
dc.subjectpolysaccharide utilization locusen_US
dc.titleA versatile genetic toolbox for Prevotella copri enables studying polysaccharide utilization systems.en_US
dc.typeArticleen_US
dc.identifier.eissn1460-2075
dc.contributor.departmentHZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.en_US
dc.identifier.journalThe EMBO journalen_US
dc.source.volume40
dc.source.issue23
dc.source.beginpagee108287
dc.source.endpage
refterms.dateFOA2022-01-11T14:39:35Z
dc.source.journaltitleThe EMBO journal
dc.source.countryEngland


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