In vitro interaction network of a synthetic gut bacterial community.
Weiss et al.pdf
OPen Access article
Microsoft Excel 2007
zipped supplemental data
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsWeiss, Anna S
Burrichter, Anna G
Durai Raj, Abilash Chakravarthy
von Strempel, Alexandra
Münch, Philipp C
Jochum, Lara M
MetadataShow full item record
AbstractA key challenge in microbiome research is to predict the functionality of microbial communities based on community membership and (meta)-genomic data. As central microbiota functions are determined by bacterial community networks, it is important to gain insight into the principles that govern bacteria-bacteria interactions. Here, we focused on the growth and metabolic interactions of the Oligo-Mouse-Microbiota (OMM12) synthetic bacterial community, which is increasingly used as a model system in gut microbiome research. Using a bottom-up approach, we uncovered the directionality of strain-strain interactions in mono- and pairwise co-culture experiments as well as in community batch culture. Metabolic network reconstruction in combination with metabolomics analysis of bacterial culture supernatants provided insights into the metabolic potential and activity of the individual community members. Thereby, we could show that the OMM12 interaction network is shaped by both exploitative and interference competition in vitro in nutrient-rich culture media and demonstrate how community structure can be shifted by changing the nutritional environment. In particular, Enterococcus faecalis KB1 was identified as an important driver of community composition by affecting the abundance of several other consortium members in vitro. As a result, this study gives fundamental insight into key drivers and mechanistic basis of the OMM12 interaction network in vitro, which serves as a knowledge base for future mechanistic in vivo studies.
CitationISME J. 2021 Dec 2. doi: 10.1038/s41396-021-01153-z. Epub ahead of print.
AffiliationBRICS, Braunschweiger Zentrum für Systembiologie, Rebenring 56, 38106 Braunschweig, Germany.
JournalThe ISME journal
The following license files are associated with this item:
- Creative Commons
- Distinct N and C Cross-Feeding Networks in a Synthetic Mouse Gut Consortium.
- Authors: Pérez Escriva P, Fuhrer T, Sauer U
- Issue date: 2022 Apr 26
- Bacterial species metabolic interaction network for deciphering the lignocellulolytic system in fungal cultivating termite gut microbiota.
- Authors: Kundu P, Mondal S, Ghosh A
- Issue date: 2022 Nov
- Short-term supplementation of celecoxib-shifted butyrate production on a simulated model of the gut microbial ecosystem and ameliorated in vitro inflammation.
- Authors: Hernandez-Sanabria E, Heiremans E, Calatayud Arroyo M, Props R, Leclercq L, Snoeys J, Van de Wiele T
- Issue date: 2020 Feb 19
- Gut-on-a-Chip for the Analysis of Bacteria-Bacteria Interactions in Gut Microbial Community: What Would Be Needed for Bacterial Co-Culture Study to Explore the Diet-Microbiota Relationship?
- Authors: Lee KW, Shin JS, Lee CM, Han HY, O Y, Kim HW, Cho TJ
- Issue date: 2023 Feb 23
- Enhanced cultured diversity of the mouse gut microbiota enables custom-made synthetic communities.
- Authors: Afrizal A, Jennings SAV, Hitch TCA, Riedel T, Basic M, Panyot A, Treichel N, Hager FT, Wong EO, Wolter B, Viehof A, von Strempel A, Eberl C, Buhl EM, Abt B, Bleich A, Tolba R, Blank LM, Navarre WW, Kiessling F, Horz HP, Torow N, Cerovic V, Stecher B, Strowig T, Overmann J, Clavel T
- Issue date: 2022 Nov 9