In vitro interaction network of a synthetic gut bacterial community.
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Authors
Weiss, Anna SBurrichter, Anna G
Durai Raj, Abilash Chakravarthy
von Strempel, Alexandra
Meng, Chen
Kleigrewe, Karin
Münch, Philipp C
Rössler, Luis
Huber, Claudia
Eisenreich, Wolfgang
Jochum, Lara M
Göing, Stephanie
Jung, Kirsten
Lincetto, Chiara
Hübner, Johannes
Marinos, Georgios
Zimmermann, Johannes
Kaleta, Christoph
Sanchez, Alvaro
Stecher, Bärbel
Issue Date
2021-12-02
Metadata
Show full item recordAbstract
A key challenge in microbiome research is to predict the functionality of microbial communities based on community membership and (meta)-genomic data. As central microbiota functions are determined by bacterial community networks, it is important to gain insight into the principles that govern bacteria-bacteria interactions. Here, we focused on the growth and metabolic interactions of the Oligo-Mouse-Microbiota (OMM12) synthetic bacterial community, which is increasingly used as a model system in gut microbiome research. Using a bottom-up approach, we uncovered the directionality of strain-strain interactions in mono- and pairwise co-culture experiments as well as in community batch culture. Metabolic network reconstruction in combination with metabolomics analysis of bacterial culture supernatants provided insights into the metabolic potential and activity of the individual community members. Thereby, we could show that the OMM12 interaction network is shaped by both exploitative and interference competition in vitro in nutrient-rich culture media and demonstrate how community structure can be shifted by changing the nutritional environment. In particular, Enterococcus faecalis KB1 was identified as an important driver of community composition by affecting the abundance of several other consortium members in vitro. As a result, this study gives fundamental insight into key drivers and mechanistic basis of the OMM12 interaction network in vitro, which serves as a knowledge base for future mechanistic in vivo studies.Citation
ISME J. 2021 Dec 2. doi: 10.1038/s41396-021-01153-z. Epub ahead of print.Affiliation
BRICS, Braunschweiger Zentrum für Systembiologie, Rebenring 56, 38106 Braunschweig, Germany.Publisher
NPGJournal
The ISME journalPubMed ID
34857933Type
ArticleLanguage
enEISSN
1751-7370ae974a485f413a2113503eed53cd6c53
10.1038/s41396-021-01153-z
Scopus Count
The following license files are associated with this item:
- Creative Commons
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