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dc.contributor.authorNasr, Sarah S
dc.contributor.authorLee, Sangeun
dc.contributor.authorThiyagarajan, Durairaj
dc.contributor.authorBoese, Annette
dc.contributor.authorLoretz, Brigitta
dc.contributor.authorLehr, Claus-Michael
dc.date.accessioned2022-01-19T12:14:21Z
dc.date.available2022-01-19T12:14:21Z
dc.date.issued2021-11-13
dc.identifier.citationPharmaceutics. 2021 Nov 13;13(11):1924. doi: 10.3390/pharmaceutics13111924.en_US
dc.identifier.issn1999-4923
dc.identifier.pmid34834339
dc.identifier.doi10.3390/pharmaceutics13111924
dc.identifier.urihttp://hdl.handle.net/10033/623148
dc.description.abstractCo-delivery of different species of protein-encoding polynucleotides, e.g., messenger RNA (mRNA) and plasmid DNA (pDNA), using the same nanocarrier is an interesting topic that remains scarcely researched in the field of nucleic acid delivery. The current study hence aims to explore the possibility of the simultaneous delivery of mRNA (mCherry) and pDNA (pAmCyan) using a single nanocarrier. The latter is based on gelatin type A, a biocompatible, and biodegradable biopolymer of broad pharmaceutical application. A core-shell nanostructure is designed with a thermally stabilized gelatin-pDNA coacervate in its center. Thermal stabilization enhances the core's colloidal stability and pDNA shielding effect against nucleases as confirmed by nanoparticle tracking analysis and gel electrophoresis, respectively. The stabilized, pDNA-loaded core is coated with the cationic peptide protamine sulfate to enable additional surface-loading with mRNA. The dual-loaded core-shell system transfects murine dendritic cell line DC2.4 with both fluorescent reporter mRNA and pDNA simultaneously, showing a transfection efficiency of 61.4 ± 21.6% for mRNA and 37.6 ± 19.45% for pDNA, 48 h post-treatment, whereas established commercial, experimental, and clinical transfection reagents fail. Hence, the unique co-transfectional capacity and the negligible cytotoxicity of the reported system may hold prospects for vaccination among other downstream applications.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectanisotropic nanogelen_US
dc.subjectcomplex coacervationen_US
dc.subjectdual loadingen_US
dc.subjectnanocarriersen_US
dc.subjectnucleic acid vaccineen_US
dc.subjectphysical cross-linkingen_US
dc.titleCo-Delivery of mRNA and pDNA Using Thermally Stabilized Coacervate-Based Core-Shell Nanosystems.en_US
dc.typeArticleen_US
dc.contributor.departmentHIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.en_US
dc.identifier.journalPharmaceuticsen_US
dc.source.volume13
dc.source.issue11
refterms.dateFOA2022-01-19T12:14:21Z
dc.source.journaltitlePharmaceutics
dc.source.countrySwitzerland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International