NUDT2 initiates viral RNA degradation by removal of 5'-phosphates.
dc.contributor.author | Laudenbach, Beatrice T | |
dc.contributor.author | Krey, Karsten | |
dc.contributor.author | Emslander, Quirin | |
dc.contributor.author | Andersen, Line Lykke | |
dc.contributor.author | Reim, Alexander | |
dc.contributor.author | Scaturro, Pietro | |
dc.contributor.author | Mundigl, Sarah | |
dc.contributor.author | Dächert, Christopher | |
dc.contributor.author | Manske, Katrin | |
dc.contributor.author | Moser, Markus | |
dc.contributor.author | Ludwig, Janos | |
dc.contributor.author | Wohlleber, Dirk | |
dc.contributor.author | Kröger, Andrea | |
dc.contributor.author | Binder, Marco | |
dc.contributor.author | Pichlmair, Andreas | |
dc.date.accessioned | 2022-01-21T16:27:58Z | |
dc.date.available | 2022-01-21T16:27:58Z | |
dc.date.issued | 2021-11-25 | |
dc.identifier.citation | Nat Commun. 2021 Nov 25;12(1):6918. doi: 10.1038/s41467-021-27239-y. | en_US |
dc.identifier.pmid | 34824277 | |
dc.identifier.doi | 10.1038/s41467-021-27239-y | |
dc.identifier.uri | http://hdl.handle.net/10033/623151 | |
dc.description.abstract | While viral replication processes are largely understood, comparably little is known on cellular mechanisms degrading viral RNA. Some viral RNAs bear a 5'-triphosphate (PPP-) group that impairs degradation by the canonical 5'-3' degradation pathway. Here we show that the Nudix hydrolase 2 (NUDT2) trims viral PPP-RNA into monophosphorylated (P)-RNA, which serves as a substrate for the 5'-3' exonuclease XRN1. NUDT2 removes 5'-phosphates from PPP-RNA in an RNA sequence- and overhang-independent manner and its ablation in cells increases growth of PPP-RNA viruses, suggesting an involvement in antiviral immunity. NUDT2 is highly homologous to bacterial RNA pyrophosphatase H (RppH), a protein involved in the metabolism of bacterial mRNA, which is 5'-tri- or diphosphorylated. Our results show a conserved function between bacterial RppH and mammalian NUDT2, indicating that the function may have adapted from a protein responsible for RNA turnover in bacteria into a protein involved in the immune defense in mammals. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Springer Nature | en_US |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | NUDT2 initiates viral RNA degradation by removal of 5'-phosphates. | en_US |
dc.type | Article | en_US |
dc.identifier.eissn | 2041-1723 | |
dc.contributor.department | HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany. | en_US |
dc.identifier.journal | Nature communications | en_US |
dc.source.volume | 12 | |
dc.source.issue | 1 | |
dc.source.beginpage | 6918 | |
dc.source.endpage | ||
refterms.dateFOA | 2022-01-21T16:27:58Z | |
dc.source.journaltitle | Nature communications | |
dc.source.country | England |