Non-Typeable Invade Choroid Plexus Epithelial Cells in a Polar Fashion.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractNon-typeable Haemophilus influenzae (NTHI) is a pathogen of the human respiratory tract causing the majority of invasive H. influenzae infections. Severe invasive infections such as septicemia and meningitis occur rarely, but the lack of a protecting vaccine and the increasing antibiotic resistance of NTHI impede treatment and emphasize its relevance as a potential meningitis causing pathogen. Meningitis results from pathogens crossing blood-brain barriers and invading the immune privileged central nervous system (CNS). In this study, we addressed the potential of NTHI to enter the brain by invading cells of the choroid plexus (CP) prior to meningeal inflammation to enlighten NTHI pathophysiological mechanisms. A cell culture model of human CP epithelial cells, which form the blood-cerebrospinal fluid barrier (BCSFB) in vivo, was used to analyze adhesion and invasion by immunofluorescence and electron microscopy. NTHI invade CP cells in vitro in a polar fashion from the blood-facing side. Furthermore, NTHI invasion rates are increased compared to encapsulated HiB and HiF strains. Fimbriae occurrence attenuated adhesion and invasion. Thus, our findings underline the role of the BCSFB as a potential entry port for NTHI into the brain and provide strong evidence for a function of the CP during NTHI invasion into the CNS during the course of meningitis.
The following license files are associated with this item:
- Creative Commons
- Capsule and fimbriae modulate the invasion of Haemophilus influenzae in a human blood-cerebrospinal fluid barrier model.
- Authors: Häuser S, Wegele C, Stump-Guthier C, Borkowski J, Weiss C, Rohde M, Ishikawa H, Schroten H, Schwerk C, Adam R
- Issue date: 2018 Oct
- [Progress toward molecular determinants of the pathogenesis of disease due to nontypeable Haemophilus influenzae].
- Authors: Tian GZ, Zhang L, Shao ZJ
- Issue date: 2009 Apr
- The PilA protein of non-typeable Haemophilus influenzae plays a role in biofilm formation, adherence to epithelial cells and colonization of the mammalian upper respiratory tract.
- Authors: Jurcisek JA, Bookwalter JE, Baker BD, Fernandez S, Novotny LA, Munson RS Jr, Bakaletz LO
- Issue date: 2007 Sep
- The adhesins of non-typeable Haemophilus influenzae.
- Authors: Osman KL, Jefferies JM, Woelk CH, Cleary DW, Clarke SC
- Issue date: 2018 Mar
- Non-typeable Haemophilus influenzae invasion and persistence in the human respiratory tract.
- Authors: Clementi CF, Murphy TF
- Issue date: 2011