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dc.contributor.authorWegele, Christian
dc.contributor.authorStump-Guthier, Carolin
dc.contributor.authorMoroniak, Selina
dc.contributor.authorWeiss, Christel
dc.contributor.authorRohde, Manfred
dc.contributor.authorIshikawa, Hiroshi
dc.contributor.authorSchroten, Horst
dc.contributor.authorSchwerk, Christian
dc.contributor.authorKarremann, Michael
dc.contributor.authorBorkowski, Julia
dc.date.accessioned2022-06-13T08:02:04Z
dc.date.available2022-06-13T08:02:04Z
dc.date.issued2020-08-10
dc.date.submitted2020-06-26
dc.identifier.pmid32785145
dc.identifier.doi10.3390/ijms21165739
dc.identifier.urihttp://hdl.handle.net/10033/623198
dc.description.abstractNon-typeable Haemophilus influenzae (NTHI) is a pathogen of the human respiratory tract causing the majority of invasive H. influenzae infections. Severe invasive infections such as septicemia and meningitis occur rarely, but the lack of a protecting vaccine and the increasing antibiotic resistance of NTHI impede treatment and emphasize its relevance as a potential meningitis causing pathogen. Meningitis results from pathogens crossing blood-brain barriers and invading the immune privileged central nervous system (CNS). In this study, we addressed the potential of NTHI to enter the brain by invading cells of the choroid plexus (CP) prior to meningeal inflammation to enlighten NTHI pathophysiological mechanisms. A cell culture model of human CP epithelial cells, which form the blood-cerebrospinal fluid barrier (BCSFB) in vivo, was used to analyze adhesion and invasion by immunofluorescence and electron microscopy. NTHI invade CP cells in vitro in a polar fashion from the blood-facing side. Furthermore, NTHI invasion rates are increased compared to encapsulated HiB and HiF strains. Fimbriae occurrence attenuated adhesion and invasion. Thus, our findings underline the role of the BCSFB as a potential entry port for NTHI into the brain and provide strong evidence for a function of the CP during NTHI invasion into the CNS during the course of meningitis.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectHaemophilus influenzaeen_US
dc.subjectNTHIen_US
dc.subjectblood–cerebrospinal fluid barrieren_US
dc.subjectchoroid plexusen_US
dc.subjecthost pathogen interactionen_US
dc.subjectmeningitisen_US
dc.titleNon-Typeable Invade Choroid Plexus Epithelial Cells in a Polar Fashion.en_US
dc.typeArticleen_US
dc.identifier.eissn1422-0067
dc.identifier.journalInternational journal of molecular sciencesen_US
dc.source.volume21
dc.source.issue16
refterms.dateFOA2022-06-13T08:02:05Z
dc.source.journaltitleInternational journal of molecular sciences
dc.source.countrySwitzerland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International