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dc.contributor.authorVermeulen, Cornelis J
dc.contributor.authorXu, Cheng-Jian
dc.contributor.authorVonk, Judith M
dc.contributor.authorTen Hacken, Nick H T
dc.contributor.authorTimens, Wim
dc.contributor.authorHeijink, Irene H
dc.contributor.authorNawijn, Martijn C
dc.contributor.authorBoekhoudt, Jeunard
dc.contributor.authorvan Oosterhout, Antoon J
dc.contributor.authorAffleck, Karen
dc.contributor.authorWeckmann, Markus
dc.contributor.authorKoppelman, Gerard H
dc.contributor.authorvan den Berge, Maarten
dc.date.accessioned2022-06-13T09:47:06Z
dc.date.available2022-06-13T09:47:06Z
dc.date.issued2020-02-06
dc.identifier.pmid31699840
dc.identifier.doi10.1183/13993003.01280-2019
dc.identifier.urihttp://hdl.handle.net/10033/623210
dc.description.abstractApproximately 40% of asthmatics experience remission of asthma symptoms. A better understanding of biological pathways leading to asthma remission may provide insight into new therapeutic targets for asthma. As an important mechanism of gene regulation, investigation of DNA methylation provides a promising approach. Our objective was to identify differences in epigenome wide DNA methylation levels in bronchial biopsies between subjects with asthma remission and subjects with persistent asthma or healthy controls.We analysed differential DNA methylation in bronchial biopsies from 26 subjects with persistent asthma, 39 remission subjects and 70 healthy controls, using the limma package. The comb-p tool was used to identify differentially methylated regions. DNA methylation of CpG-sites was associated to expression of nearby genes from the same biopsies to understand function.Four CpG-sites and 42 regions were differentially methylated between persistent asthma and remission. DNA methylation at two sites was correlated i n cis with gene expression at ACKR2 and DGKQ Between remission subjects and healthy controls 1163 CpG-sites and 328 regions were differentially methylated. DNA methylation was associated with expression of a set of genes expressed in ciliated epithelium.CpGs differentially methylated between remission and persistent asthma identify genetic loci associated with resolution of inflammation and airway responsiveness. Despite the absence of symptoms, remission subjects have a DNA methylation profile that is distinct from that of healthy controls, partly due to changes in cellular composition, with a higher gene expression signal related to ciliated epithelium in remission versus healthy controls.en_US
dc.language.isoenen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleDifferential DNA methylation in bronchial biopsies between persistent asthma and asthma in remission.en_US
dc.typeArticleen_US
dc.identifier.eissn1399-3003
dc.identifier.journalThe European respiratory journalen_US
dc.source.volume55
dc.source.issue2
refterms.dateFOA2022-06-13T09:47:06Z
dc.source.journaltitleThe European respiratory journal
dc.source.countryEngland


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International