Methylation of Salmonella Typhimurium flagella promotes bacterial adhesion and host cell invasion.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsHorstmann, Julia A
Lokareddy, Ravi K
Hughes, Kelly T
Grassl, Guntram A
Stradal, Theresia E B
MetadataShow full item record
AbstractThe long external filament of bacterial flagella is composed of several thousand copies of a single protein, flagellin. Here, we explore the role played by lysine methylation of flagellin in Salmonella, which requires the methylase FliB. We show that both flagellins of Salmonella enterica serovar Typhimurium, FliC and FljB, are methylated at surface-exposed lysine residues by FliB. A Salmonella Typhimurium mutant deficient in flagellin methylation is outcompeted for gut colonization in a gastroenteritis mouse model, and methylation of flagellin promotes bacterial invasion of epithelial cells in vitro. Lysine methylation increases the surface hydrophobicity of flagellin, and enhances flagella-dependent adhesion of Salmonella to phosphatidylcholine vesicles and epithelial cells. Therefore, posttranslational methylation of flagellin facilitates adhesion of Salmonella Typhimurium to hydrophobic host cell surfaces, and contributes to efficient gut colonization and host infection.
The following license files are associated with this item:
- Creative Commons
- Enhancement of host immune responses by oral vaccination to Salmonella enterica serovar Typhimurium harboring both FliC and FljB flagella.
- Authors: Eom JS, Seok Kim J, Im Jang J, Kim BH, Young Yoo S, Hyeon Choi J, Bang IS, Lee IS, Keun Park Y
- Issue date: 2013
- The YrbE phospholipid transporter of <i>Salmonella enterica</i> serovar Typhi regulates the expression of flagellin and influences motility, adhesion and induction of epithelial inflammatory responses.
- Authors: Verma S, Prescott RA, Ingano L, Nickerson KP, Hill E, Faherty CS, Fasano A, Senger S, Cherayil BJ
- Issue date: 2020 May 3
- Flagellar phase variation of Salmonella enterica serovar Typhimurium contributes to virulence in the murine typhoid infection model but does not influence Salmonella-induced enteropathogenesis.
- Authors: Ikeda JS, Schmitt CK, Darnell SC, Watson PR, Bispham J, Wallis TS, Weinstein DL, Metcalf ES, Adams P, O'Connor CD, O'Brien AD
- Issue date: 2001 May
- Flagellin phase-dependent swimming on epithelial cell surfaces contributes to productive Salmonella gut colonisation.
- Authors: Horstmann JA, Zschieschang E, Truschel T, de Diego J, Lunelli M, Rohde M, May T, Strowig T, Stradal T, Kolbe M, Erhardt M
- Issue date: 2017 Aug
- Absence of all components of the flagellar export and synthesis machinery differentially alters virulence of Salmonella enterica serovar Typhimurium in models of typhoid fever, survival in macrophages, tissue culture invasiveness, and calf enterocolitis.
- Authors: Schmitt CK, Ikeda JS, Darnell SC, Watson PR, Bispham J, Wallis TS, Weinstein DL, Metcalf ES, O'Brien AD
- Issue date: 2001 Sep