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dc.contributor.authorHeimes, A.
dc.contributor.authorBrodhagen, J.
dc.contributor.authorWeikard, R.
dc.contributor.authorBecker, D.
dc.contributor.authorMeyerholz, M. M.
dc.contributor.authorPetzl, W.
dc.contributor.authorZerbe, H.
dc.contributor.authorSchuberth, H. J.
dc.contributor.authorHoedemaker, M.
dc.contributor.authorSchmicke, M.
dc.contributor.authorEngelmann, S.
dc.contributor.authorKühn, C.
dc.date.accessioned2022-06-20T09:08:21Z
dc.date.available2022-06-20T09:08:21Z
dc.date.issued2020-07-01
dc.date.submitted2019-09-19
dc.identifier.citationHeimes A, Brodhagen J, Weikard R, Becker D, Meyerholz MM, Petzl W, Zerbe H, Schuberth HJ, Hoedemaker M, Schmicke M, Engelmann S, Kühn C. Cows selected for divergent mastitis susceptibility display a differential liver transcriptome profile after experimental Staphylococcus aureus mammary gland inoculation. J Dairy Sci. 2020 Jul;103(7):6364-6373. doi: 10.3168/jds.2019-17612. Epub 2020 Apr 16. PMID: 32307160.en_US
dc.identifier.issn00220302
dc.identifier.pmid32307160
dc.identifier.doi10.3168/jds.2019-17612
dc.identifier.urihttp://hdl.handle.net/10033/623236
dc.descriptionInfection and inflammation of the mammary gland, and especially prevention of mastitis, are still major challenges for the dairy industry. Different approaches have been tried to reduce the incidence of mastitis. Genetic selection of cows with lower susceptibility to mastitis promises sustainable success in this regard. Bos taurus autosome (BTA) 18, particularly the region between 43 and 59 Mb, harbors quantitative trait loci (QTL) for somatic cell score, a surrogate trait for mastitis susceptibility. Scrutinizing the molecular bases hereof, we challenged udders from half-sib heifers having inherited either favorable paternal haplotypes for somatic cell score (Q) or unfavorable haplotypes (q) with the Staphylococcus aureus pathogen. RNA sequencing was used for an in-depth analysis of challenge-related alterations in the hepatic transcriptome. Liver exerts highly relevant immune functions aside from being the key metabolic organ. Hence, a holistic approach focusing on the liver enabled us to identify challenge-related and genotype-dependent differentially expressed genes and underlying regulatory networks. In response to the S. aureus challenge, we found that heifers with Q haplotypes displayed more activated immune genes and pathways after S. aureus challenge compared with their q half-sibs. Furthermore, we found a significant enrichment of differentially expressed loci in the genomic target region on BTA18, suggesting the existence of a regionally acting regulatory element with effects on a variety of genes in this region. © 2020 American Dairy Science Associationen_US
dc.description.abstractInfection and inflammation of the mammary gland, and especially prevention of mastitis, are still major challenges for the dairy industry. Different approaches have been tried to reduce the incidence of mastitis. Genetic selection of cows with lower susceptibility to mastitis promises sustainable success in this regard. Bos taurus autosome (BTA) 18, particularly the region between 43 and 59 Mb, harbors quantitative trait loci (QTL) for somatic cell score, a surrogate trait for mastitis susceptibility. Scrutinizing the molecular bases hereof, we challenged udders from half-sib heifers having inherited either favorable paternal haplotypes for somatic cell score (Q) or unfavorable haplotypes (q) with the Staphylococcus aureus pathogen. RNA sequencing was used for an in-depth analysis of challenge-related alterations in the hepatic transcriptome. Liver exerts highly relevant immune functions aside from being the key metabolic organ. Hence, a holistic approach focusing on the liver enabled us to identify challenge-related and genotype-dependent differentially expressed genes and underlying regulatory networks. In response to the S. aureus challenge, we found that heifers with Q haplotypes displayed more activated immune genes and pathways after S. aureus challenge compared with their q half-sibs. Furthermore, we found a significant enrichment of differentially expressed loci in the genomic target region on BTA18, suggesting the existence of a regionally acting regulatory element with effects on a variety of genes in this region. © 2020 American Dairy Science Associationen_US
dc.description.sponsorshipLandwirtschaftliche Rentenbanken_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesVolume 103, Issue 7, July 2020, Pages 6364-6373en_US
dc.rightsAttribution-NonCommercial 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.subjectBTA18en_US
dc.subjectliver transcriptomeen_US
dc.subjectmastitisen_US
dc.subjectRNAseqen_US
dc.subjectsomatic cell scoreen_US
dc.titleCows selected for divergent mastitis susceptibility display a differential liver transcriptome profile after experimental Staphylococcus aureus mammary gland inoculationen_US
dc.typeArticleen_US
dc.typeOtheren_US
dc.identifier.eissn15253198
dc.contributor.departmentLeibniz Institute for Farm Animal Biology (FBN), Institute of Genome Biology, Dummerstorf, 18196, GermanyClinic for Ruminants with Ambulatory and Herd Health Services, Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-University Munich, Oberschleißheim, 85764, GermanyImmunology Unit, University of Veterinary Medicine Hannover, Hannover, 30559, GermanyClinic for Cattle, University of Veterinary Medicine Hannover, Hannover, 30173, GermanyFaculty of Natural Sciences III, Martin-Luther Universität Halle-Wittenberg, Halle, 06120, GermanyTechnical University Braunschweig, Institute for Microbiology, Braunschweig, 38023, GermanyHelmholtz Centre for Infection Research, Microbial Proteomics, Braunschweig, 38124, GermanyAgricultural and Environmental Faculty, University Rostock, Rostock, 18059, Germanyen_US
dc.identifier.journalJournal of Dairy Scienceen_US
dc.identifier.eid2-s2.0-85083320906
dc.identifier.scopusidSCOPUS_ID:85083320906
dc.identifier.piiS0022030220302745
dc.source.volume103
dc.source.issue7
dc.source.beginpage6364
dc.source.endpage6373
refterms.dateFOA2020-09-19T00:00:00Z
dc.source.journaltitleJournal of Dairy Science


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