ANALYTISCHE CHARAKTERISIERUNG VON NICHT-PROTEINARTIGEN BEGLEITSTOFFEN IN EINEM SCP (METHYLOMONAS CLARA)

Abstract

Analytical profile of non protein compounds in a SCP (M. clara) In different batches of a single cell protein (Methylomonas clara, Fa. Hoechst) the lipid fraction as well as the nucleic acid fraction were characterised by analytical means. 65 % of the extracted lipid fraction were phospholipids, 15 % were neutral lipids and 20 % were glycolipids. The unsaponifiable part was 1,4 %, polyhydroxybutyrate (PHP) was not detected. Unusual fatty acids as cyclopropane- or cyclopropene fatty acids were absent. The fatty acid profile revealed that 80-90 3 were Cıg-fatty acids. The content of free bases in the nucleic acid fraction was surprisingly high, this is probably due to a cleavage of RNA during the technical processing of the bacterial product. Switching from batch fermentation to continuous fermentation resulted in a significant decrease of the RNA content. By a special two stage extraction procedure a protein concentrate is obtained which containes only small amounts of lipids and nucleic acids. This product is of much higher nutritional value.