Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Issue Date
1975Submitted date
2023-01-31
Metadata
Show full item recordAbstract
Purified capsular material of Myxococcus xanthus has previously been shown to be composed mainly of N-acetylgalactosamine, glucose and glycine. Investigations carried out during the last two years have demonstrated that UDP Gal NAc and UDP Glc are the major precursors. Biosynthesis of capsule was studied by measuring the incorporation of 140 _upp Gal NAc, 344-UDP Glc and Su-glycine into TCA insoluable material. All three compounds were incorporated more rapidly than into crude extracts prepared from 2 hr glycerol induced myxospores that in similar extracts prepared from vegetative cells. Preliminary studies indicated that incorporation of UDP Gal NAc was stimulated by UDP Glc and to a lesser degree by glycine and ATP. Similarly, incorporation of UDP Glc was stimulated by UDP Gal NAc, glycine and ATP. Both of the transferase activities were found exclusively in the 30,000 x g pellet, which were used for further studies. The glycine incorporating activity remained in the supernatant. Both transferase activities were linear with time for 1 hr and with protein concentration between 0.1 - 1.0 mg/ml. The incorporation of UDP Glc and UDP Gal NAc were absolutely dependent upon divalent cations and were inhibited 40 % by 100 pg/ml Bacitracin. In the absence of ATP, the incorporation of UDP Glc was stimulated by UDP Gal NAc, but UDP Gal NAc incorporation was not stimulated by various concentrations of UDP Gic. In both cases, the label was incorporated into the 30,000 x g pellet, from which it could be recovered as radioactive glucose and galactosamine following acid hydrolysis and paper chromatography.Citation
2nd International Symposium on the Biology of Myxobacteria, 7Affiliation
Department of Microbiology, Tel Aviv University Tel Aviv, IsraelType
Book chapterconference paper
Language
enCollections
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International