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dc.contributor.authorCaruthers, M. H.
dc.contributor.authorDubendorff, J. W.
dc.contributor.authorde Haseth, P. L.
dc.contributor.authorTang, J.-Y.
dc.contributor.authorProsser, K.
dc.contributor.authorRosendahl, M. S.
dc.date.accessioned2023-04-19T10:29:09Z
dc.date.available2023-04-19T10:29:09Z
dc.date.issued1987
dc.date.submitted2023-04-19
dc.identifier.citationChemical synthesis in molecular biology, 1 ffen_US
dc.identifier.issn0930-4320
dc.identifier.urihttp://hdl.handle.net/10033/623354
dc.description.abstractCurrent methods for synthesizing DNA utilize protected deoxynucleoside phosphoramidites as synthons and a glass or silica gel solid support as the synthesis matrix. Syntheses are extremely rapid (5-10 minutes per cycle) and lead to high yields of deoxyoligonucleotides containing 20-100 mononucleotides each. Using these methods to synthesize modified DNAs, the interaction of Z. coli RNA polymerase and cI repressor with lambda Pp promoter has been studied. Results identify certain 5-methyl groups located in the -35 region of the promoter and Opl that are important protein recognition sites. These results correlate quite nicely to previous research with Jac repressor. Additional data suggest possible insights regarding the formation of open promoter complexes.en_US
dc.language.isoenen_US
dc.relation.ispartofseriesGBF Monographs, Vol. 8en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titlePROTEIN-DNA RECOGNITION: THE INTERACTION OF Jac, cI AND Z. coli RNA POLYMERASE WITH OPERATORS AND PROMOTERSen_US
dc.typeBook chapteren_US
dc.typeconference paperen_US
dc.contributor.departmentDepartment of Chemistry, University of Colorado, Boulder, Campus Box 215, Boulder, CO 80309, USAen_US
dc.identifier.journalChemical synthesis in molecular biologyen_US
refterms.dateFOA2023-04-19T10:29:09Z


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Attribution-NonCommercial-ShareAlike 4.0 International
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