Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Authors
Wang, DebaoIssue Date
1987Submitted date
2023-04-19
Metadata
Show full item recordAbstract
By means of a combination of chemical and enzymatic methods, we have synthesized an RNA molecule which has the same chemical composition (i.e. it contains all the common as well as the modified nucleotides) and structure as the natural yeast alanyl tRNA, elucidated by R.W. Holley et.al. and modified by others, and which is biologically active (1). In the presence of rat liver aminoacyl-tRNA synthetase, the synthetic tRNA can be charged with alanine and the incorporation of alanine from the charged tRNA molecule into proteins is readily demonstrated in a rabbit reticulocyte ‘ lysate system.Citation
Chemical synthesis in molecular biology, 43 ffAffiliation
Shanghai Institute of Biochemistry, Academia Sinica Shanghai, ChinaType
Book chapterconference paper
Language
enSeries/Report no.
GBF Monographs, Vol. 8ISSN
0930-4320Collections
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International