Culture Fluorescence Studies for Process Monitoring and Control in Biotechnology

Abstract

Efficient "control of biological processes requires che measurement of various biological parameters. The on-line measurement of NADH- dependent culture fluorescence gives a direct insight into the microorganisms during cultivation. A miniaturized microfluorometer with special fibre optics for the simultaneous detection of two different wavelengths was constructed and applied for process monitoring. In addition an Ingold prototyp fluorometer ( Dr. Ingold AG, Switzerland) was also studied.