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dc.contributor.authorArnold, Mark A.
dc.date.accessioned2023-09-06T10:09:43Z
dc.date.available2023-09-06T10:09:43Z
dc.date.issued1987
dc.date.submitted2023-09-06
dc.identifier.citationBiosensors International Workshop 1987, 223 - 227en_US
dc.identifier.isbn0-89573-683-7
dc.identifier.isbn3-527-26801-4
dc.identifier.issn0930-4320
dc.identifier.urihttp://hdl.handle.net/10033/623473
dc.description.abstractFiber optic biosensing probes consist of an immobilized biocatalytic layer at the distil tip of an optical fiber device. In the presence of the bioanalyte, the biocatalyst generates an optical signal which is measured and related to the bioanalyte concentration. Three new classes of fiber optic biosensors are presented. The first class is based on the immobilization of a deaminating enzyme at the tip of an fiber optic ammonia sensor. The first fiber optic urea sensor has been successfully demonstrated by immobilizing urease at the tip of an optimized fiber optic ammonia sensor. The second type of fiber optic biosensor is based on the immobilization of a dehydrogenase enzyme and the fluorometric detection of reduced nicotinamide adenine dinucleotide (NADH) at the distil tip of an optical fiber bundle. The feasibility of this NADH-based biosensor strategy has been successfully demonstrated with biosensors for lactate and pyruvate. Finally, a novel biosensor arrangement is introduced where the biocatalyst is separated from the sample solution by a gas-permeable membrane. This "internal enzyme" approach has been demonstrated with an ethanol sensor in which alcohol dehydrogenase is employed as the enzyme and the production of NADH is detected fluorometrically. Relative merits and future prospects for these three types of fiber optic biosensors are discussed.en_US
dc.language.isoenen_US
dc.publisherGBF - Gesellschaft für Biotechnologische Forschungen_US
dc.relation.ispartofseriesGBF Monographs, Volume 10en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleDEVELOPMENT OF BIOSENSORS USING OPTICAL FIBERSen_US
dc.typeBook chapteren_US
dc.typeconference paperen_US
dc.contributor.departmentDepartment of Chemistry, University of Iowa, Iowa City, Iowa 52242en_US
dc.identifier.journalBiosensors International Workshop 1987en_US
refterms.dateFOA2023-09-06T10:09:44Z


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