Einfluß des Drucks auf immobilisierte Enzyme

Abstract

Pressure shows a distinct effect on the activity and stability of enzymes in solution. We observed for Trypsin an increase of activity of 39,8 % at 55 °C. The investigations were made continously by using a tubular reactor. Enzymes. immobilized on Eupergit C®, which is a water-insoluble carrier, behave in a similar manner but with smaller influence of pressure. In opposite to Trypsin in solution, the carrierbound enzyme had a denaturation point of 60 °C (Trypsin in solution: 50 °C). Also the stability rose with pressure. Similar results were obtained using immobilized Penicillin-Acylase. At 40°C and 300 bar no decrease of activity could be observed after an incubation time of 2 hours. This high stability leads to to an increased lifetime of the catalyst which is promotive for technical use.