ENZYME AND IMMUNE ELECTRODES FOR THE CLINICAL LABORATORY

Abstract

Different variants of glucose oxidase electrodes and equipment for it have been successfully used for years in health institutions in the GDR. In addition to glucose in blood, urine glucose can also be measured, where there is a similarity to the hexokinase method. For this however, instead of measuring the maximum transformation velocity of the H205 oxidation flow, the oxygen uptake must be determined. The possibility of carrying out an enzyme electrochemical dry test is demonstrated using a simple device, which serves to measure glucose with enzyme films. In spite of its good analytical quality, an enzyme-free amperometric measurement of uric acid has only attained little practical use. The reasons for this are drug interactions. Studies on analyte diffusion through the membrane to the electrode demonstrate this. Enzyme activities from ‘380i: 50¢nM oh sat <i emae in the uricase membrane yield electrodes with good measuring characteristics. Catalase electrodes make a simple performance of the indicator reaction in enzyme immuno-assays possible. A distinct improvement of the analytical quality could not be observed when compared to the photometer method.