CONTINUOUS GLUCOSE MONITORING IN SUBCUTANEOUS TISSUE AND BLOOD WITH AN AMPEROMETRIC ENZYME ELECTRODE

Abstract

As we have described previously, an amperometric enzyme electrode was developed, based on the detection of hydrogen peroxide generated by the oxidation of glucose (Kerner et al., Horm.Metab.Res.Suppl.20: 8-13,1988). This needle-type electrode was implanted into sc.tissue of sheep. It is shown that the sensor current is closely related to the course of blood glucose. For measurement of glucose in blood comparable to the Biostator, an electrode was further modified and integrated into a flow-chamber system. In vitro experiments demonstrated its qualification. Monitoring of blood glucose was performed over 24 hours.