Show simple item record

dc.contributor.authorCammann, Karl
dc.contributor.authorJahn, Kerstin
dc.contributor.authorRohen, Anja
dc.contributor.authorWeiß, Thomas
dc.contributor.authorWilken, Hildegard
dc.contributor.authorWinter, Babette
dc.date.accessioned2023-12-13T12:05:45Z
dc.date.available2023-12-13T12:05:45Z
dc.date.issued1989
dc.date.submitted2023-12-13
dc.identifier.citationBiosensors : applications in medicine, environmental protection and process control, 201 - 211en_US
dc.identifier.isbn3527280324
dc.identifier.isbn0895739550
dc.identifier.issn0930-4320
dc.identifier.urihttp://hdl.handle.net/10033/623569
dc.description.abstractNAD*/NADH und NADP*/NADPH systems, which are important coenzymes, are studied with respect to their determination by electrochemical or optical methods relevant for biosensors. Concerning their sensitivity and linearity of the calibration curves, similar results were obtained, when the technique of Flow-Injection Analysis (FIA) was used in conjunction with an amperometric flow-through cell and when a fiberoptic device measuring the fluorescence of NAD(P)H was employed. In both cases the limit of detection was around 1 nmol/l and the calibration curves showed two linear ranges. As a consequence an optical glucosesensor based on this technique resulted in a correspondingly high sensitivity compared to a classical needle type amperometric device based on the glucose-oxidase (GOD) method. Otherwise, the superb sensitivity achievable by the amperometric NADH oxidation could by used for a sensitive formaldehyde FIA method yielding a limit of detection in the sub-nmol/l range. In addition, a reagentless fiber optic lactate sensor based on lactate-dehydrogenase (LDH) and a reversible redox-dye is described and also a novel potentiometric immunosensor based on the principle of selectivity modulation of ion-selective membranes.en_US
dc.language.isoenen_US
dc.publisherGBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweigen_US
dc.relation.ispartofseriesGBF monographs ; Volume 13en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleCOMPARISON OF ELECTROCHEMICAL WITH OPTICAL BIOSENSORSen_US
dc.typeBook chapteren_US
dc.typeconference paperen_US
dc.contributor.departmentLehrstuhl für analytische Chemie Westfälische Wilhelms-Universität Wilhelm-Klemm-StraBe 8 D-4400 Munster, GFRen_US
dc.identifier.journalBiosensors : applications in medicine, environmental protection and process control, 1989en_US
refterms.dateFOA2023-12-13T12:05:47Z


Files in this item

Thumbnail
Name:
Cammann201.pdf
Size:
4.877Mb
Format:
PDF
Description:
PDF

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-ShareAlike 4.0 International
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International