PRINCIPLES OF SIGNAL GENERATION AND OF COUPLING TO OPTICAL FIBERS: DYNAMIC FLUORESCENCE BIOSENSORS

Kasche, V.; Käärd, A.; Renken, E.; Grewe, H.; Jansen, K.; Ulrich, R.

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Authors

Kasche, V.
Käärd, A.
Renken, E.
Grewe, H.
Jansen, K.
Ulrich, R.

Issue Date

1989

Submitted date

2024-01-16

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Abstract

We have investigated in detail the transport processes and the enzyme catalyzed reactions that govern the generation of a fluorescence signal in dynamic biosensors, using a biosensor for penicillin G with a measurings range of 0,1 — 100 mM penicillin G. The optical read-out of fluorescence changes is analyzed by considering the optical power budget. We show the limitations to the final output signal set by the properties of the source, fibers, optics, filters, detectors, noise, and fluctuations.

Citation

Biosensors : applications in medicine, environmental protection and process control, 233 - 242

Affiliation

Technische Universität Hamburg - Harburg, Postfach 90 14 03, D-2100 Hamburg 90

Type

Book chapter
conference paper

Language

Series/Report no.

GBF monographs ; Volume 13

ISSN

0930-4320

ISBN

3527280324
0895739550

Collections

GBF Series' articles

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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International