BIOSENSORS ON-LINE IN HPLC

Abstract

Analysis in clinical chemistry, food chemistry or biochemistry are often concerned with the difficulty to determine special substances with high sensitivity (e.g. in the pmole-range). Often the commonly known methods for detection are not sensitive enough or complicated sample preparations are necessary. To solve such problems in analysis we have developed on-line-Biosensors for HPLC. The Biosensor in this system is defined by two parts: immobilized enzymes creating H202 and a Pt-working electrode in an electrochemical detector. We present here two examples of the method. Sensitive and rapid determination of acetylcholine and choline and of hypoxanthine and xanthine. By combining the high resolving power of HPLC with the specificity of immobilized enzymes and the high sensitivity of electrochemical detection, HPLC/Biosensor-technology is a simple and economic method for many problens in analysis and will have widespread further applications.