FLOW INJECTION ZYMOGRAPHY FOR THE ON-LINE DETECTION OF ENZYMEACTIVITY

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Authors
Heine-Dobbernack, Elke
Künnecke, Wolfgang
Kalisz, Henryk M.
Schmid, Rolf D.
Issue Date
1989
Submitted date
2024-01-16

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Abstract
new method - Flow Injection Zymography - has been developed for the detection of enzymeactivity during purification procedures usingflow injection analysis (FIA). Four oxidases and a lipase were used as model enzymes. The method involves frequent sampling ofeluents via a flow injection system and enables swift localization of the active fractions. Furthermore, the dilution technique of zone sampling enables estimation of the purification effect.
Citation
Biosensors : applications in medicine, environmental protection and process control, 295 - 302
Affiliation
GBF- Gesellschaft fiir Biotechnologische Forschung, Abteilung fiir Enzymtechnologie, Mascheroder Weg 1, 3300 Braunschweig, FRG.
Publisher
GBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweig
Journal
Biosensors : applications in medicine, environmental protection and process control, 1989
URI
http://hdl.handle.net/10033/623584
Type
Book chapter
conference paper
Language
en
Series/Report no.
GBF monographs ; Volume 13
ISSN
0930-4320
ISBN
3527280324
0895739550
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