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FLOW INJECTION IMMUNOANALYSIS (FIIA) FOR THE DETERMINATION OF PESTICIDES IN WATER

dc.contributor.authorStöcklein, W.
dc.contributor.authorKrämer, P.
dc.contributor.authorSchmid, Rolf D.
dc.date.accessioned2024-01-16T10:40:12Z
dc.date.available2024-01-16T10:40:12Z
dc.date.issued1989
dc.date.submitted2024-01-16
dc.identifier.citationBiosensors : applications in medicine, environmental protection and process control, 307 - 312en_US
dc.identifier.isbn3527280324
dc.identifier.isbn0895739550
dc.identifier.issn0930-4320
dc.identifier.urihttp://hdl.handle.net/10033/623587
dc.description.abstractThe principle of a heterogeneous competitive enzyme immunoassay was applied to a flow injection system. This flow injection immunoassay (FIIA) can be run automatically. Antibodies to the triazine herbicide atrazin were immobilized on membranes. Antibody containing membranes were mounted in a cross-flow reactor and changed after each assay. In the assay atrazin competed with an atrazin-peroxidase conjugate for the antibody binding sites. The product of the enzyme reaction was measured fluorimetrically. One assay is completed within 15 minutes. Sensitivities of the FIIA proved similar to the ELISA and depended mainly on the properties of the antibodies and conjugation strategies. The potential and limitations of the FIIA system will be discussed.en_US
dc.language.isoenen_US
dc.publisherGBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweigen_US
dc.relation.ispartofseriesGBF monographs ; Volume 13en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleFLOW INJECTION IMMUNOANALYSIS (FIIA) FOR THE DETERMINATION OF PESTICIDES IN WATERen_US
dc.typeBook chapteren_US
dc.typeconference paperen_US
dc.contributor.departmentGBF - Gesellschaft für Biotechnologische Forschung, Abt. für Enzymtechnologie, Mascheroder Weg 1, D-3300 Braunschweig, FRGen_US
dc.identifier.journalBiosensors : applications in medicine, environmental protection and process control, 1989en_US
refterms.dateFOA2024-01-16T10:40:13Z


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Attribution-NonCommercial-ShareAlike 4.0 International
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International