A NOVEL ENZYMATIC ASSAY METHOD FOR HYPOXANTHINE

Kim, Jong Min; Suzuki, Masayasu; Schmid, Rolf D.

dc.contributor.author	Kim, Jong Min
dc.contributor.author	Suzuki, Masayasu
dc.contributor.author	Schmid, Rolf D.
dc.date.accessioned	2024-01-16T12:37:02Z
dc.date.available	2024-01-16T12:37:02Z
dc.date.issued	1989
dc.date.submitted	2024-01-16
dc.identifier.citation	Biosensors : applications in medicine, environmental protection and process control, 425 - 428	en_US
dc.identifier.isbn	3527280324
dc.identifier.isbn	0895739550
dc.identifier.issn	0930-4320
dc.identifier.uri	http://hdl.handle.net/10033/623604
dc.description.abstract	We developed a highly sensitive and rapid enzymatic assay for hypoxanthine with a Clark oxygen electrode as sensor. In the presence of sodium sulfite, oxidation of hypoxanthine by milk xanthine oxidase caused very rapid and increased oxygen consumption in excess of the stoichiometric requirement for hypoxanthine oxidation. Hypoxanthine from 0.5 to 10 uM can be assayed within a few minutes by addition of 25 mM sodium sulfite to the reaction mixture. This assay proved to be more than 10 times more sensitive and much more rapid than control method without sulfite.	en_US
dc.language.iso	en	en_US
dc.publisher	GBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweig	en_US
dc.relation.ispartofseries	GBF monographs ; Volume 13	en_US
dc.rights	Attribution-NonCommercial-ShareAlike 4.0 International	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-sa/4.0/	*
dc.title	A NOVEL ENZYMATIC ASSAY METHOD FOR HYPOXANTHINE	en_US
dc.type	Book chapter	en_US
dc.type	conference paper	en_US
dc.contributor.department	GBF-Gesellschaft fuer Biotechnologische Forschung mbH Mascheroder Weg 1, 3300 Braunschweig, F.R.G.	en_US
dc.identifier.journal	Biosensors : applications in medicine, environmental protection and process control, 1989	en_US
refterms.dateFOA	2024-01-16T12:37:03Z