On Line Determination of Glucose Concentration in Animal Cell Cultures Based on Chemiluminescent Detection Coupled with Flow Injection Analysis

Abstract

A flow injection analysis (FIA) system for the on-line determination of glucose in animalcell cultures is described. The hydrogen peroxide generated in the enzyme reaction is determined via a highly sensitive chemiluminescent reaction with luminol. Based on the measurement of the maximum emitted light intensity, the system was able to analyse glucose in a linear range of 10° to 5x 102 M glucose concentration, with an r.s.d. of 3% at the 1 mM level (5 measurements). The enzyme reactor used was stable for more than 4 weeks in continous operation. The FIA developed wasable to analyse up to 20 samples per hour. The system has been sucessfully applied to on-line monitoring of glucose concentration during an animal cell culture, designed for the production of human antithrombin III factor. Results obtained with the FIA system were compared with off-line results, obtained with a Yellow Springs Instrument Company Model27 (YSI).