ON-LINE MEASUREMENT OF INTRACELLULAR ENZYME ACTIVITY DURING FERMENTATION USING PERMEABILIZED CELLSIN A FIA SYSTEM

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Authors
Blankenstein, G.
Kula, M.-R.
Issue Date
1991
Submitted date
2024-01-31

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Abstract
A novel method for determination of intracellular enzyme activity in yeast cells has been deviced. After permeabilization it is possible to determine the activity of NAD-dependent formate dehydrogenase from Candida boidinii based on stopped flow injection techniques combined with fluorimetric detection. Yeast cells were made permeable using the surfactant Digitonin in combination with Triton X—100.
Citation
Flow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 85 - 88
Affiliation
Institut für Enzymtechnologie Heinrich-Heine-Universität Düsseldorf, FRG
Publisher
GBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweig
Journal
Flow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 1991
URI
http://hdl.handle.net/10033/623618
Type
Book chapter
conference paper
Language
en
Series/Report no.
GBF monographs ; Volume 14
ISSN
0930-4320
ISBN
1560811218
3527282491
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