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dc.contributor.authorBlankenstein, G.
dc.contributor.authorKula, M.-R.
dc.date.accessioned2024-01-31T10:46:33Z
dc.date.available2024-01-31T10:46:33Z
dc.date.issued1991
dc.date.submitted2024-01-31
dc.identifier.citationFlow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 85 - 88en_US
dc.identifier.isbn1560811218
dc.identifier.isbn3527282491
dc.identifier.issn0930-4320
dc.identifier.urihttp://hdl.handle.net/10033/623618
dc.description.abstractA novel method for determination of intracellular enzyme activity in yeast cells has been deviced. After permeabilization it is possible to determine the activity of NAD-dependent formate dehydrogenase from Candida boidinii based on stopped flow injection techniques combined with fluorimetric detection. Yeast cells were made permeable using the surfactant Digitonin in combination with Triton X—100.en_US
dc.language.isoenen_US
dc.publisherGBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweigen_US
dc.relation.ispartofseriesGBF monographs ; Volume 14en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleON-LINE MEASUREMENT OF INTRACELLULAR ENZYME ACTIVITY DURING FERMENTATION USING PERMEABILIZED CELLSIN A FIA SYSTEMen_US
dc.typeBook chapteren_US
dc.typeconference paperen_US
dc.contributor.departmentInstitut für Enzymtechnologie Heinrich-Heine-Universität Düsseldorf, FRGen_US
dc.identifier.journalFlow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 1991en_US
refterms.dateFOA2024-01-31T10:46:35Z


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Attribution-NonCommercial-ShareAlike 4.0 International
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