ONLINE MONITORING OF L-LYSINE IN TECHNICAL CHROMATOGRAPHY BY FLOW INJECTION ANALYSIS(FIA)
Abstract
An enzymatic assay is presented which is highly selective for L-lysine and‘is based on FIA techniques combined with photometric detection. L-Iysine-a-oxidase (EC 1.4.3.14) from Trichodermaviride and horseradish peroxidase were coimmobilized on VA Epoxy resin in an analytical enzyme reactor, which wasincorporated in the FIA system. 30 samples hot, sample volume2 ul, linearity range 1-16 mM L-lysine, correlation coeficient 0.998, limit of detection 1 mM and 0.5 % RSD. The enzyme Cartridge can be used for several thousand measurements over a period some of several months. The methodis applicable for monitoring in technical ion exchange chromatography. The values correlate well with the ones determined with an amino acid analyser. Breakthrough point detection is possible and productspecific control may now be achieved.Citation
Flow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 101 - 105Affiliation
4 Institute of Enzyme Technology, Heinrich Heine Universität Düsseldorf (located in the Forschungszentrum KFA Jülich), P.O.Box 2050, D-5170 Jülich, FRG Present adress: B.Braun Diessel Biotech GmbH, P.O.Box 120, D-3508 Melsungen, FRG © Degussa AG, D-4802 Halle - Kinsebeck, FRGType
Book chapterconference paper
Language
enSeries/Report no.
GBF monographs ; Volume 14ISSN
0930-4320ISBN
15608112183527282491
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