DEVELOPMENT OF ONE-SHOT FLOW INJECTION ANALYSIS WITH IMMOBILIZED ENZYME COLUMN AND ITS APPLICATION TO CLINICAL ANALYSIS

Abstract

The availavility of enzymes as the reagents for clinical analysis has been wellknown. The enzymes can be used not only in the form of solution but also in the form of immobilized enzyme reactor. The determinations of glucose and uric acid in serum by chemiluminometry after the production of hydrogen peroxide by the respective oxidase are presented. Newly chemiluminometric methods for ammonia and NAD(P)H using an enzyme column reactor consisting of both immobilized L-glutamate dehydrogenase and L-glutamate oxidase, and its application to other enzymatic analyses that give ammonia and NAD(P)H as a final signal, e.g. urea and magnesium, are described. A compact automated analyzer which can analyse constituents in biological fluids using these enzymatic methods with a small sample volume and in a short time has been developed. The instrument is composed of an one-shot FIA system, a FIA system of new type, equipped with chemiluminometric detection and a immobilized enzyme column reactor used in combination. The one-shot FIA system is also described.