ENZYME-CATALYZED DETERMINATION OF 2-OXOISOCAPROATE BY FLOW-INJECTION ANALYSIS AND ITS APPLICATION TO THE MONITORING FOR MAPLE SYRUP URIN DISEASE

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Authors
Tauschensky, S.
Hummel, W.
Schadewaldt, P.
Wendel, U.
Issue Date
1991
Submitted date
2024-01-31

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Abstract
A simple and fast method for the determination of 2—oxoisocaproate in plasma samples by use of NAD*-dependent D-2-hydroxyisocaproate dehydrogenase (Hic-DH) from Lactobacillus casei ssp. is described. The enzyme is immobilized on aminopropyl-CPG and applicated in a flow-injection system with detection of NADH absorbance. Applicability is demonstrated by comparative measurement of 2-0xoisocaproate levels in plasma samples of patients with maple syrup urine disease by the photometric method and the flow-injection analysis.
Citation
Flow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 145 - 148
Affiliation
Institut fiir Enzymtechnologie Diabetes Forschungsinstitut und Institut für Physiologische Chemie II 3Kinderklinik Heinrich—Heine Universität Düsseldorf FRG
Publisher
GBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweig
Journal
Flow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 1991
URI
http://hdl.handle.net/10033/623625
Type
Book chapter
conference paper
Language
en
Series/Report no.
GBF monographs ; Volume 14
ISSN
0930-4320
ISBN
1560811218
3527282491
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