ELIMINATION OF INTERFERENCES IN GLUCOSE DETERMINATION IN BLOOD SERUM USING FLOW-INJECTION AMPEROMETRY
Abstract
A flow-injection system with anodic amperometric detection for glucose determination in human blood serum samples was optimized by a comparison of various arrangements of enzyme immobilization and elimination of interferences such as ascorbic acid, urea and uric acid. The best results were obtained using a compact membrane biosensor in wall-jet flow-through detector, where Pt disc electrode was covered with evaporated Nafion layer, then a polyester membrane with immobilized glucose oxidase and another protective polyester membrane. In the optimized conditions a linear response up to 25mM glucose was observed with detection limit 200uM glucose and sampling rate 120 hr-1 for 20p1 sample volume. Results of flow injection glucose determination in human serum samples were compared with Beckman Glucose Analyser 2 and Kone Dynamic analyser.Citation
Flow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 159 - 163Affiliation
Department of Chemistry, University of Warsaw, Pasteura l, 02-093 Warsaw, Poland; Department of Analytical Chemistry, Abo Akademi University, SF-20500 Turku, FinlandType
Book chapterconference paper
Language
enSeries/Report no.
GBF monographs ; Volume 14ISSN
0930-4320ISBN
15608112183527282491
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