STEREOSELECTIVE DETERMINATIONOF L- AND D-AMINO ACIDS IN SERUM BY THEUSE OF IMMOBILIZED ENZYME REACTORSIN FIA

Abstract

A flow injection method containing immobilized enzyme reactors (IMERs) ; L-amino acid oxidase (L-AAO), D-aminoacid oxidase (D-AAO)and Horse radish peroxidase (HRP) for the determination of L-and D-amino acids has be en developed. Enzymatic conversion efficiencies for twenty L-and D-amino acids are given. The linear range of the detecteable amino acids was around two orders of magnitude. The sample frequency was 19 h” with a sample volume of 50 yl. The methodis used for the stereoselective determination of L-and D-aminoacids in the presence of L-aminoacids in serum.