SIMULTANEOUS FLOW INJECTION DETERMINATION OF MALATE AND ETHANOL BASED ON OXYGEN CONSUMPTION WHITH DEHYDROGENASE AND OXIDASE REACTORS
Abstract
The simultaneous determination system of L-malate and ethanol was developed by using two enzyme reactors in parallel and a single oxygen electrode. NADH formed in the reaction of malate dehydrogenase was regenerated to NAD with dissolved oxygen using vitamin K; and diaphorase. Ethanol was determined by use of alcohol oxidase. When a sample solution was simultaneously injected to two reactors, a train of two peaks corresponding to ethanol and L-malate appeared in an FIA-gran. The peak current was linearly related to ethanol and L-malate concentration in the range 18-50 mM and 0.09-0.9 mM, respectively. The present system was applicable to the determination of ethanol and L-malate in wine.Citation
Flow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 203 - 208Affiliation
Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Fukuoka 812, JAPANType
Book chapterconference paper
Language
enSeries/Report no.
GBF monographs ; Volume 14ISSN
0930-4320ISBN
15608112183527282491
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