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dc.contributor.authorGorton, Lo
dc.contributor.authorBardheim, M.
dc.contributor.authorBremle, G.
dc.contributor.authorCsöregi, E.
dc.contributor.authorPersson, B.
dc.contributor.authorPettersson, G.
dc.date.accessioned2024-02-07T14:33:47Z
dc.date.available2024-02-07T14:33:47Z
dc.date.issued1991
dc.date.submitted2024-02-07
dc.identifier.citationFlow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 305 - 314en_US
dc.identifier.isbn1560811218
dc.identifier.isbn3527282491
dc.identifier.issn0930-4320
dc.identifier.urihttp://hdl.handle.net/10033/623644
dc.description.abstractAmperometric biosensors based on two different reaction mechanisms are presented. Common to both types is the combination of a selective catalytic reaction that can be followed amperometrically at 0 mV vs SCE and below. One type is based on the chemical modification of carbon pastes with a dehydrogenase, the necessary cofactor NAD’, and a redox mediator. In the presence of the enzyme substrate NADH will be produced. The high overvoltage for the electrochemical oxidation of the NADH is decreased by the addition of the redox mediator to the paste. The redox mediators used are phenoxazine derivatives making the electrocatalytic oxidation of NADH possible at 0 mV vs SCE and below. A glucose sensor based on glucose dehydrogenase is described. Another type is based on the co-immobilization of a hydrogen peroxide producing oxidase with horse radish peroxidase on the surface of heat-treated graphite. The detection is based on an apparent direct electron transfer from the electrode to the immobilized peroxidase starting at +600 mV and reaching a maximum at about O mV vs SCE. The co-immobilized enzyme layer is stabilised by the addition of bovine serum albumin and glutaraldehyde to the reaction mixture. A glucose sensor based on glucose oxidase is presented.en_US
dc.language.isoenen_US
dc.publisherGBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweigen_US
dc.relation.ispartofseriesGBF monographs ; Volume 14en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleAMPEROMETRIC BIOSENSORS BASED ON IMMOBILIZED ENZYMES AND CHEMICALLY MODIFIED ELECTRODESen_US
dc.typeBook chapteren_US
dc.typeconference paperen_US
dc.contributor.departmentDept. of Analytical Chemistry, University of Lund, P. O. Box 124, S- 221 00 Lund, Sweden.en_US
dc.identifier.journalFlow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 1991en_US
refterms.dateFOA2024-02-07T14:33:48Z


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Attribution-NonCommercial-ShareAlike 4.0 International
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