Design and Application of a new Two-Channel FIA

Abstract

A new two-channel pneumatic slider valve was constructed by modification of a commercial valve. This valve simplifies the flow injection analysis of two components in a sample stream utilizing general advantages of pneumatically driven slider valves as there are small sampling volume(22 ul), high frequencies of analyses (25 sec), simple construction and maintenance, high reliability, low costs and,finally, ease of multiplication, as many slider valves can be operated by one pneumatic actuator, if installed in an appropriate housing. The performanceofthe valve is exemplified by analysis of a gelfiltration with penicillin acylase, alkaline phosphatase and molecular weight markers. The new designed valve, integrated in FIA systems, enables the independentanalysis of two different components or, alternatively, the analysis of one componentby different assays.