CRYSTALLIZATION OF GASTRIC LIPASES
Abstract
Gastric lipases are pH resistant enzymes with a molecular weight of ca 50,000, including 15% sugars. Every glyco-variant from different species have been purified by ief, and submitted to crystallization, using parameters found by an incomplete factorial analysis method. All attempts were successful, but most crystals were unsuitable to X-ray studies due to huge cell dimensions. Dog gastric lipase crystals, the most suitable for X-ray study (pH 6.8, 12% PEG 6000, 20°C; P2242 :182A, 211A, 98 A), contain ca 8 molecules in the asymetric unit , and therefore diffract to only 4A on lab sources.Citation
Lipases : structure, mechanism and genetic engineering, 27 - 30Affiliation
Laboratoire de Cristallographie et Cristallisation des Macromolécules Biologiques, Faculté de Médecine Nord, Bvd. P.Dramard, 13326 MARSEILLE CEDEX15 , France. Hervé Moreau,Frédéric Carriére and Robert Verger Centre de Biochimie et de Biologie Moléculaire, 31 chemin J. Aiguier 13009 MARSEILLE,FranceType
Book chapterconference paper
Language
enSeries/Report no.
GBF monographs ; Volume 16ISSN
0930-4320ISBN
156081165X3527283323
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