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CRYSTALLIZATION AND PRELIMINARY X-RAY ANALYSIS OF A LIPASE FROM A SPECIES OF PSEUDOMONAS

dc.contributor.authorSarma, Ragupathy
dc.contributor.authorDauberman, Judy
dc.contributor.authorPoulose, A. J.
dc.contributor.authorVan Bielen, Jan
dc.contributor.authorPower, Scott
dc.contributor.authorShew, Brian
dc.contributor.authorGray, Greg
dc.contributor.authorNorton, Shirley
dc.contributor.authorVan Kimmenade, Anita
dc.contributor.authorJarnagin, Alisha
dc.contributor.authorAshizawa, Eunice
dc.contributor.authorBoston, Matthew
dc.contributor.authorRequadt, Carol
dc.contributor.authorKolattakudy, P. E.
dc.contributor.authorBott, Richard
dc.date.accessioned2024-03-13T09:30:38Z
dc.date.available2024-03-13T09:30:38Z
dc.date.issued1991
dc.date.submitted2024-03-13
dc.identifier.citationLipases : structure, mechanism and genetic engineering, 71 - 76en_US
dc.identifier.isbn156081165X
dc.identifier.isbn3527283323
dc.identifier.issn0930-4320
dc.identifier.urihttp://hdl.handle.net/10033/623700
dc.description.abstractA lipase from a species of Pseudomonas has been cloned and expressed in E. coli. Variants of this lipase have been generated, using sitespecific mutagenesis, that have significantly altered k,,,, K, and substrate specificity. We have undertaken to determine the threedimensional structure of this enzyme using X-ray crystallography. Crystals have been obtained and these crystals diffract to 2.5 Angstrom resolution. We are now in the process of evaluating heavy atom derivatives to be used to improve the phase information used to calculate electron density maps.en_US
dc.language.isoenen_US
dc.publisherGBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweigen_US
dc.relation.ispartofseriesGBF monographs ; Volume 16en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleCRYSTALLIZATION AND PRELIMINARY X-RAY ANALYSIS OF A LIPASE FROM A SPECIES OF PSEUDOMONASen_US
dc.typeBook chapteren_US
dc.typeconference paperen_US
dc.contributor.departmentGenencor International, So. San Francisco, CA 94080 USA; S.U.N.Y. at Stony Brook, Stony Brook, NY 11794 USA; 0hio State University, Columbus OH 43210 USAen_US
dc.identifier.journalLipases : structure, mechanism and genetic engineering, 1991en_US
refterms.dateFOA2024-03-13T09:30:39Z


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