COMPARATIVE STUDY ON PRIMARY STRUCTURES OF TWO LIPASES FROM GEOTRICHUM CANDIDUM

Abstract

Geotrichum(G.) candidum produces two extracellular lipases I and II, the lipase I being the predominant component. The lipase I and II cDNAs were cloned, and their nucleotide sequences were determined. The nucleotide sequences included the N- and Cterminal amino acid sequences and the partial amino acid sequences of the lipases. The mature lipases I and II were of the same length, their overall identity being 84%, Furthermore, four Cys residues were completely conserved, which may participate in the formation of disulfide bridges. A homology search indicated that the G. candidum lipases and Candida cylindracea lipase are homologous enzymes, and that they are members of the cholinesterase family.