CLONING, SEQUENCING AND REGULATION OF THE LIPASE GENE FROM PSEUDOMONASSP. M-12-33

Abstract

Thelipase gene from Pseudomonas sp. M-12-33 was cloned. The cloned DNA was 2.9 Kb in length, which was essential for production of the lipase and contained two open reading frames, lipA and lipX. The lipA gene was supposed to comprise 1092 nucleotides and give a preproprotein of 364 aminoacids which was then processed to a mature lipase protein of 320 amino acids. On the other hand, the lipX gene was assumedto code a protein of 344 amino acids concerned with someregulation of the enzyme production.