USTILAGO MAYDIS LIPOLYTIC ENZYMES: CHARACTERIZATION AND PARTIAL PURIFICATION

Abstract

The crude lipase preparation of Ustilago maydis ATCC 14826 (after growth on coconut oil) was studied with respect to hydrolysis and esterification potential, resp., as well as to purification of lipolytic enzymes. Concerning substrate specifity (hydrolysis) among triglycerides short chain substances were cleaved to an higher extent than long chain or unsaturated compounds. Obvious inhibition of lipase activity was observed when additional amounts of linoleic or linolenic acid were used during hydrolysis of coconut oil. After Amberlite XAD-2 immobilization and transfer into n-hexane the wax ester synthesis potential was confirmed. The purification of crude lipase preparation by chromatographic methods led to two lipolytic enzymes.