Show simple item record

dc.contributor.authorJaeger, Karl-Erich
dc.contributor.authorWohlfarth, Susanne
dc.contributor.authorWinkler, Ulrich K.
dc.date.accessioned2024-03-27T10:16:54Z
dc.date.available2024-03-27T10:16:54Z
dc.date.issued1991
dc.date.submitted2024-03-27
dc.identifier.citationLipases : structure, mechanism and genetic engineering, 381 - 384en_US
dc.identifier.isbn156081165X
dc.identifier.isbn3527283323
dc.identifier.issn0930-4320
dc.identifier.urihttp://hdl.handle.net/10033/623744
dc.description.abstractLipase of Pseudomonas aeruginosa was excreted in form of high M,- aggregates consisting of protein and lipopolysaccharide. Solubilization and isoelectric focusing in the presence of the zwitterionic detergent CHAPS yielded in an electrophoretically pure lipase protein of M, 29 kDa with an isoelectric point of 5.9. Charge shift electrophoresis showed that lipase was an amphiphilic protein, its activity was dependent on the presence of detergent. Lipase cleaved a variety of different substrates showing no positional specificity. The lipase gene was cloned and sequenced revealing the lipase consensus sequence Gly-His-Ser-His-Gly. Polyclonal antibodies were raised against purified lipase having a titer of 1400 and a detection limit in the picogram range.en_US
dc.language.isoenen_US
dc.publisherGBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweigen_US
dc.relation.ispartofseriesGBF monographs ; Volume 16en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleEXTRACELLULAR LIPASE OF PSEUDOMONAS AERUGINOSAen_US
dc.typeBook chapteren_US
dc.typeconference paperen_US
dc.contributor.departmentRuhr-Universität, Lehrstuhl Biologie der Mikroorganismen, D-4630 Bochum, FRGen_US
dc.identifier.journalLipases : structure, mechanism and genetic engineering, 1991en_US
refterms.dateFOA2024-03-27T10:16:55Z


Files in this item

Thumbnail
Name:
Jaeger381.pdf
Size:
2.204Mb
Format:
PDF
Description:
PDF

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-ShareAlike 4.0 International
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International